Figure 1: High Performance Liquid Chromatogram of BSA (bovine serum albumin) tryptic peptides
Applications of LC-MS (Liquid Chromatography – Mass Spectromery) in Chemical and Biochemical Sciences
Aldol condensation - leading to aldol or ketol which can undergo dehydration to produce the alpha,beta-unsaturated aldehyde or ketone
Aldol Condensation – Acid Catalyzed
Base catalyzed dehydration of aldol or ketol leading to alpha,beta-unsaturated carbonyl compound.
Aldol Condensation – Base Catalyzed
Competitive antagonism of tetrahydrofolate biosynthesis by sulfonamides and diaminopyridines
Mechanism of Action of Tetrahydrofolate Synthesis Inhibitors – Sulfonamides and Diaminopyrimidines
Mechanism of Action of Quinolones and Fluoroquinolones by inhibition of DNA gyrase (DNA topoisomerase)