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Analytical Chemistry Archive

The Visible Spectrum with VIBGYOR (Violet, Indigo, Blue, Green, Yellow, Orange, Red)

Introduction to the Electromagnetic Spectrum and Spectroscopy

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Different transitions between the bonding and anti-bonding electronic states when light energy is absorbed in UV-Visible Spectroscopy.

Ultraviolet-Visible (UV-Vis) Spectroscopy – Principle

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A diagrammatic representation of the hypothetical situation to derive the Beer-Lambert law containing a sample of path length b through which monochromatic light is passed.

Ultraviolet-Visible (UV-Vis) Spectroscopy – Derivation of Beer-Lambert Law

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Figure A: Shows the difference in deviations in absorbance when values are obtained at maximum wavelength of absorbance (band A) vs other wavelengths of absorbance (band B). Figure B: shows the deviations in Beer-Lambert law due to observations made at wavelengths other than lambda max.

Ultraviolet-Visible (UV-Vis) Spectroscopy – Limitations and Deviations of Beer-Lambert Law

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Transoid and Cisoid Dienes with substituents highlighted in red.

Ultraviolet-Visible (UV-Vis) Spectroscopy – Woodward-Fieser Rules to Calculate Wavelength of Maximum Absorption (Lambda-max) of Conjugated Dienes and Polyenes

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highlighting substituents in alpha,beta and alpha,beta-gamma,delta- unsaturated carbonyl compounds

Ultraviolet-Visible (UV-Vis) Spectroscopy – Woodward-Fieser Rules to Calculate Wavelength of Maximum Absorption (Lambda-max) of Conjugated Carbonyl Compounds

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Steroidal Molecule solved using Woodward-Fieser rules

Ultraviolet-Visible (UV-Vis) Spectroscopy – Sample Problems Using Woodward-Fieser Rules

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pKa scale for acidity and basicity

Henderson–Hasselbalch Equation: Derivation of pKa and pKb

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Thin Layer Chromatography (TLC): Principle (with Animation)

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Principle of Reversed-Phase Chromatography HPLC/UPLC (with Animation)

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Principle and Mechanisms of Reversed-Phase Ion-Pairing Chromatography (with Animation)

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Animation for the Principle of Fluorescence and UV-Visible Absorbance

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Wavelength of maximum absorption for all-trans-lycophene

Ultraviolet-Visible (UV-Vis) Spectroscopy – Fieser-Kuhn Rules to Calculate Wavelength of Maximum Absorption (Lambda-max) of Polyenes (with Sample Problems)

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Applications and Examples of Henderson Hasselbalch Equation

Applications and Example Problems Using Henderson–Hasselbalch Equation

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