Description - A 3D animation illustrating the process by which a protein is mass-produced using spliced DNA and bacterial replication using Plasmids. DNA recombinant technology is used to produce proteins using bacteria. The bacteria being easier to grow than actual animals, proteins can be mass produced using them. This animation shows how exactly using the DNA from which the protein can be made, a plasmid is generated which is a recombinant DNA plasmid, which when reintroduced into the bacteria promotes the bacteria to synthesize the protein.

Since the animation is a long, we suggest that you CLICK THE PLAY BUTTON IN THE MIDDLE and give it a while to download. After it has completed the video once, we suggest that you rewind and playback the second time as it will go faster.
 
NOTE - The animation shows Pst1 Restriction Enzyme ( represented by the scissor) which acts as a scissor to cut the plasmid DNA at the Pst1 Restriction site to form sticky ends of DNA. The cDNA of the protein molecule of interest is then glued to the sticky ends using the Enzyme DNA Ligase (represented by the glue). After this the recombinant DNA plasmid is then put in the bacteria to grow in two different methods as shown in the animation.